Modifying Enzymes
New England Biolabs, Inc. NotI-HF® – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GC^GGCC_GC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. Taq DNA Ligase Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Taq DNA Ligase. Taq DNA Ligase requires NAD+ as a cofactor which is incorporated into the buffer. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Sigma Aldrich Fine Chemicals Biosciences Exonuclease I 10 U/muL Cyt
Exonuclease I (Exo I) fromnbspE. colinbspis an Mg2nbspion dependent exonuclease.
ABclonal Technology T4 RNA Ligase 1
T4 RNA ligase 1 catalyzes the formation of a 3 to 5 phosphodiester bond by joining the 5 phosphate end of a nucleotide to the 3 hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.
Worthington Biochemical Corporation ENDOPROTEINASE GLU-C 1MG
Protease, S. Aureus (Endoproteinase Glu-C), Code: STAP, Minimum activity: 500U/mg dry weight, A lyophilized powder, Store at 2 deg. to 8 deg.C, 1mg
Supply Solutions Roche - Nuclease S7; Micrococcal nuclease, from Staphylococcus aureus; pkg of 15,000 U
Roche - Nuclease S7; Micrococcal nuclease, from Staphylococcus aureus; pkg of 15,000 U
Supply Solutions Roche - Neuraminidase(Sialidase); from Arthrobacter ureafaciens; pkg of 1 U (100 ul)
Roche - Neuraminidase(Sialidase); from Arthrobacter ureafaciens; pkg of 1 U (100 ul)
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 1KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 1KU
Sigma Aldrich Fine Chemicals Biosciences Lipase from porcine pancreas | 9001-62-1 | MFCD00131509 | 100g
Lipase from porcine pancreas | 9001-62-1 | MFCD00131509 | 100g
Medchemexpress LLC Ribonuclease T1, Aspergillus oryzae | 9026-12-4 | 11.2 kDa monomer | 10 KU
Ribonuclease T1, Aspergillus oryzae | 9026-12-4 | 11.2 kDa monomer | 10 KU
United States Biological Corporation ZYMOLYASE 100T 50MG
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg
Carnabio Usa Inc WNK4/10UG/CATALYTIC
WNK4, Catalytic domain, Wild type, Amino Acid 1-444, NP_115763.2, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. Msz Exonuclease I - 1000 units
A DNA specific exonuclease isolated from Methylocaldum szegediense that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3 prime to 5 prime direction with optimal activity between 45 and 60 degrees C. Isolated from a strain of E. coli which carries the cloned pMC45-1 gene from Methylocaldum szegediense.
Worthington Biochemical Corporation Protease S. aureus Sequencing Grade
Protease S. aureus Sequencing Grade
Sigma Aldrich Fine Chemicals Biosciences Oleoyl 13C18 coenzyme A li10MG
Oleoyl 13C18 coenzyme A li10MG