Modifying Enzymes
STEMCELL Technologies Hyaluronidase, Size: 50000 U
Hyaluronidase is a polysaccharidase consisting of a single polypeptide chain of 450 amino acid residues and contains four disulfide bonds. Hyaluronidase is glycosylated and contains 5% mannose and 2.17% glucosamine (Borders & Raftery). Hyaluronidase cleaves endo-N-acetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate A and C to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in connective tissues. Hyaluronidase is frequently used with other proteases such as collagenase.
Sigma Aldrich Fine Chemicals Biosciences Endoproteinase Asp-N from Pseudomonas fragi mutant strain suitable for protein sequencing, lyophilized powder | 9001-92-7 | MFCD02253021 | 1VL
Endoproteinase Asp-N from Pseudomonas fragi mutant strain suitable for protein sequencing, lyophilized powder | 9001-92-7 | MFCD02253021 | 1VL
Sigma Aldrich Fine Chemicals Biosciences Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
Sigma Aldrich Fine Chemicals Biosciences Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 250ML
Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 250ML
Sigma Aldrich Fine Chemicals Biosciences beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
Santa Cruz Biotechnology Lambda Phosphatase, 20000 units
Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins; also active on phosphorylated histidine residues. 1 mL of 10X Reaction buffer and 1 mL of 10X MnCl2 included with each size. Lambda Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The enzyme is inhibited by vanadate ions and can be heat inactivated at 65° C for 1 hour in the presence of 50 mM EDTA.
American Research Products Inc AMERICAN RESEARCH PRODUCTS INC
5000613139 HUMAN DNA LIGASE 3 ELISA KIT
Sigma Aldrich Fine Chemicals Biosciences Glutamate Dehydrogenase (GlDH), 3000 units
Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album, 39450-01-6, MFCD00132129, 100 mg
Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology, Synonym: Endopeptidase K. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria. Determination of enzyme localization on membranes. Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Sigma Aldrich Fine Chemicals Biosciences Papain from papaya latex, 9001-73-4, MFCD00131791, 25 mg
Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.
New England Biolabs, Inc. Bacteroides Heparinase I – 600 units
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Supply Solutions Roche - Proteinase K,Recomb.,Pcr Grd.Lyo.,100 Mg
Roche - Proteinase K,recomb.,PCR Grd.lyo.,100 mg; Lyophilized from Pichia pastoris; pkg of 100 mg (03115879001) pkg of 25 mg (03115836001) pkg of 2 x 250 mg (03115801001) pkg of 4 x 250 mg (0311582001)
Supply Solutions Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)
Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)